Journal: ACS Omega
Article Title: Fluorescent Labeling of Ultrahigh Molecular Weight Polyacrylamide Polymers in Microemulsion Systems
doi: 10.1021/acsomega.4c10667
Figure Lengend Snippet: Atomic force microscopy (AFM) analysis of HPAM polymers before and after EDC/NHS treatment. (a) Dry-phase AFM image of HPAM polymer molecules deposited on the surface. Scale bar: 2 μm. (b) Enlarged view of a single HPAM polymer molecule, showing the circular 1D projection of the polymer random coil. Scale bar: 100 nm. (c) Lateral size distribution of HPAM polymer molecules, fitted to a Gaussian distribution with a mean diameter of 0.18 μm. (d) Dry-phase AFM image of the HPAM-EDC-NHS complex after 2 h of incubation, revealing elongated and branched features representing bundled polymer chains. Scale bar: 1 μm.
Article Snippet: Atomic Force Microscopy (AFM) was conducted using a Bruker Dimension Icon instrument operating in intermittent contact mode.
Techniques: Microscopy, Polymer, Incubation